18 November 2015 Integrated femtosecond stimulated Raman scattering and two-photon fluorescence imaging of subcellular lipid and vesicular structures
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Abstract
The primary goal of this study is to demonstrate that stimulated Raman scattering (SRS) as a new imaging modality can be integrated into a femtosecond (fs) nonlinear optical (NLO) microscope system. The fs sources of high pulse peak power are routinely used in multimodal nonlinear microscopy to enable efficient excitation of multiple NLO signals. However, with fs excitations, the SRS imaging of subcellular lipid and vesicular structures encounters significant interference from proteins due to poor spectral resolution and a lack of chemical specificity, respectively. We developed a unique NLO microscope of fs excitation that enables rapid acquisition of SRS and multiple two-photon excited fluorescence (TPEF) signals. In the
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xuesong Li, Xuesong Li, Wen Jiun Lam, Wen Jiun Lam, Zhe Cao, Zhe Cao, Yan Hao, Yan Hao, Qiqi Sun, Qiqi Sun, Sicong He, Sicong He, Ho Yi Mak, Ho Yi Mak, Jianan Y. Qu, Jianan Y. Qu, } "Integrated femtosecond stimulated Raman scattering and two-photon fluorescence imaging of subcellular lipid and vesicular structures," Journal of Biomedical Optics 20(11), 110501 (18 November 2015). https://doi.org/10.1117/1.JBO.20.11.110501 . Submission:
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