6 October 2015 Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing
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Abstract
We propose a new portable imaging configuration that can double the field of view (FOV) of existing off-axis interferometric imaging setups, including broadband off-axis interferometers. This configuration is attached at the output port of the off-axis interferometer and optically creates a multiplexed interferogram on the digital camera, which is composed of two off-axis interferograms with straight fringes at orthogonal directions. Each of these interferograms contains a different FOV of the imaged sample. Due to the separation of these two FOVs in the spatial-frequency domain, they can be fully reconstructed separately, while obtaining two complex wavefronts from the sample at once. Since the optically multiplexed off-axis interferogram is recorded by the camera in a single exposure, fast dynamics can be recorded with a doubled imaging area. We used this technique for quantitative phase microscopy of biological samples with extended FOV. We demonstrate attaching the proposed module to a diffractive phase microscopy interferometer, illuminated by a broadband light source. The biological samples used for the experimental demonstrations include microscopic diatom shells, cancer cells, and flowing blood cells.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Pinhas Girshovitz, Pinhas Girshovitz, Irena Frenklach, Irena Frenklach, Natan T. Shaked, Natan T. Shaked, } "Broadband quantitative phase microscopy with extended field of view using off-axis interferometric multiplexing," Journal of Biomedical Optics 20(11), 111217 (6 October 2015). https://doi.org/10.1117/1.JBO.20.11.111217 . Submission:
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