17 November 2015 Oral cancer screening: serum Raman spectroscopic approach
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Serum Raman spectroscopy (RS) has previously shown potential in oral cancer diagnosis and recurrence prediction. To evaluate the potential of serum RS in oral cancer screening, premalignant and cancer-specific detection was explored in the present study using 328 subjects belonging to healthy controls, premalignant, disease controls, and oral cancer groups. Spectra were acquired using a Raman microprobe. Spectral findings suggest changes in amino acids, lipids, protein, DNA, and β-carotene across the groups. A patient-wise approach was employed for data analysis using principal component linear discriminant analysis. In the first step, the classification among premalignant, disease control (nonoral cancer), oral cancer, and normal samples was evaluated in binary classification models. Thereafter, two screening-friendly classification approaches were explored to further evaluate the clinical utility of serum RS: a single four-group model and normal versus abnormal followed by determining the type of abnormality model. Results demonstrate the feasibility of premalignant and specific cancer detection. The normal versus abnormal model yields better sensitivity and specificity rates of 64 and 80%; these rates are comparable to standard screening approaches. Prospectively, as the current screening procedure of visual inspection is useful mainly for high-risk populations, serum RS may serve as a useful adjunct for early and specific detection of oral precancers and cancer.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Aditi Sahu, Aditi Sahu, Suyash Dhoot, Suyash Dhoot, Amandeep Singh, Amandeep Singh, Sharada Sawant, Sharada Sawant, Nikhila Nandakumar, Nikhila Nandakumar, Sneha Talathi-Desai, Sneha Talathi-Desai, Mandavi Garud, Mandavi Garud, Sandeep Pagare, Sandeep Pagare, Sanjeeva Srivastava, Sanjeeva Srivastava, Sudhir Nair, Sudhir Nair, Pankaj Chaturvedi, Pankaj Chaturvedi, C. Murali Krishna, C. Murali Krishna, } "Oral cancer screening: serum Raman spectroscopic approach," Journal of Biomedical Optics 20(11), 115006 (17 November 2015). https://doi.org/10.1117/1.JBO.20.11.115006 . Submission:

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