30 November 2015 Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses
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Abstract
We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Aisada Uchugonova, Aisada Uchugonova, Hans Georg Breunig, Hans Georg Breunig, Ana Batista, Ana Batista, Karsten König, Karsten König, } "Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses," Journal of Biomedical Optics 20(11), 115008 (30 November 2015). https://doi.org/10.1117/1.JBO.20.11.115008 . Submission:
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