11 December 2015 Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy
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Abstract
Herein, the optical adequacy of a tumor model prepared with tumor cells grown on the chorioallantoic membrane (CAM) of a chicken egg is evaluated as an alternative to the mouse tumor model to assess the optimal irradiation conditions in photodynamic therapy (PDT). The optical properties of CAM and mouse tumor tissues were measured with a double integrating sphere and the inverse Monte Carlo technique in the 350- to 1000-nm wavelength range. The hemoglobin and water absorption bands observed in the CAM tumor tissue (10 eggs and 10 tumors) are equal to that of the mouse tumor tissue (8 animals and 8 tumors). The optical intersubject variability of the CAM tumor tissues meets or exceeds that of the mouse tumor tissues, and the reduced scattering coefficient spectra of CAM tumor tissues can be equated with those of mouse tumor tissues. These results confirm that the CAM tumor model is a viable alternative to the mouse tumor model, especially for deriving optimal irradiation conditions in PDT.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Norihiro Honda, Norihiro Honda, Yoichiro Kariyama, Yoichiro Kariyama, Hisanao Hazama, Hisanao Hazama, Takuya Ishii, Takuya Ishii, Yuya Kitajima, Yuya Kitajima, Katsushi Inoue, Katsushi Inoue, Masahiro Ishizuka, Masahiro Ishizuka, Tohru Tanaka, Tohru Tanaka, Kunio Awazu, Kunio Awazu, } "Optical properties of tumor tissues grown on the chorioallantoic membrane of chicken eggs: tumor model to assay of tumor response to photodynamic therapy," Journal of Biomedical Optics 20(12), 125001 (11 December 2015). https://doi.org/10.1117/1.JBO.20.12.125001 . Submission:
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