16 December 2015 Quantitative Raman spectral changes of the differentiation of mesenchymal stem cells into islet-like cells by biochemical component analysis and multiple peak fitting
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Abstract
Mesenchymal stem cells (MSCs) differentiate into islet-like cells, providing a possible solution for type I diabetes treatment. To search for the precise molecular mechanism of the directional differentiation of MSC-derived islet-like cells, biomolecular composition, and structural conformation information during MSC differentiation, is required. Because islet-like cells lack specific surface markers, the commonly employed immunostaining technique is not suitable for their identification, physical separation, and enrichment. Combining Raman spectroscopic data, a fitting accuracy-improved biochemical component analysis, and multiple peaks fitting approach, we identified the quantitative biochemical and intensity change of Raman peaks that show the differentiation of MSCs into islet-like cells. Along with increases in protein and glycogen content, and decreases in deoxyribonucleic acid and ribonucleic acid content, in islet-like cells relative to MSCs, it was found that a characteristic peak of insulin (665  cm−1) has twice the intensity in islet-like cells relative to MSCs, indicating differentiation of MSCs into islet-like cells was successful. Importantly, these Raman signatures provide useful information on the structural and pathological states during MSC differentiation and help to develop noninvasive and label-free Raman sorting methods for stem cells and their lineages.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xin Su, Xin Su, Shaoyin Fang, Shaoyin Fang, Daosen Zhang, Daosen Zhang, Qinnan Zhang, Qinnan Zhang, Yingtian He, Yingtian He, Xiaoxu Lu, Xiaoxu Lu, Shengde Liu, Shengde Liu, Liyun Zhong, Liyun Zhong, } "Quantitative Raman spectral changes of the differentiation of mesenchymal stem cells into islet-like cells by biochemical component analysis and multiple peak fitting," Journal of Biomedical Optics 20(12), 125002 (16 December 2015). https://doi.org/10.1117/1.JBO.20.12.125002 . Submission:
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