24 February 2015 Intravital live cell triggered imaging system reveals monocyte patrolling and macrophage migration in atherosclerotic arteries
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Abstract
Intravital multiphoton imaging of arteries is technically challenging because the artery expands with every heartbeat, causing severe motion artifacts. To study leukocyte activity in atherosclerosis, we developed the intravital live cell triggered imaging system (ILTIS). This system implements cardiac triggered acquisition as well as frame selection and image registration algorithms to produce stable movies of myeloid cell movement in atherosclerotic arteries in live mice. To minimize tissue damage, no mechanical stabilization is used and the artery is allowed to expand freely. ILTIS performs multicolor high frame-rate two-dimensional imaging and full-thickness three-dimensional imaging of beating arteries in live mice. The external carotid artery and its branches (superior thyroid and ascending pharyngeal arteries) were developed as a surgically accessible and reliable model of atherosclerosis. We use ILTIS to demonstrate GFP −/− GFP/+ YFP
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Sara McArdle, Sara McArdle, Grzegorz Chodaczek, Grzegorz Chodaczek, Nilanjan Ray, Nilanjan Ray, Klaus Ley, Klaus Ley, } "Intravital live cell triggered imaging system reveals monocyte patrolling and macrophage migration in atherosclerotic arteries," Journal of Biomedical Optics 20(2), 026005 (24 February 2015). https://doi.org/10.1117/1.JBO.20.2.026005 . Submission:
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