13 February 2015 Tip-enhanced fluorescence with radially polarized illumination for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA
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Abstract
A tip nanobiosensor for monitoring DNA replication was presented. The effects of excitation power and polarization on tip-enhanced fluorescence (TEF) were assessed with the tip immersed in fluorescein isothiocyanate solution first. The photon count rose on average fivefold with radially polarized illumination at 50 mW. We then used polymerase-functionalized tips for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA. The amplicon-SYBR® Green I complex was detected and compared to real-time loop-mediated isothermal amplification. The signals of the reaction using 4 and 0.004  ng/μl templates were detected 10 and 30 min earlier, respectively. The results showed the potential of TEF in developing a nanobiosensor for real-time DNA amplification.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Shih-Chung Wei, Tsung-Liang Chuang, Da-Shin Wang, Hui-Hsin Lu, Frank X. Gu, Kung-Bin Sung, Chii-Wann Lin, "Tip-enhanced fluorescence with radially polarized illumination for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA," Journal of Biomedical Optics 20(2), 027005 (13 February 2015). https://doi.org/10.1117/1.JBO.20.2.027005 . Submission:
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