Differential laser-induced perturbation Raman spectroscopy: a comparison with Raman spectroscopy for analysis and classification of amino acids and dipeptides

Erman K. Oztekin; Sarah E. Smith; David W. Hahn

doi:10.1117/1.JBO.20.4.047006

23 April 2015 Differential laser-induced perturbation Raman spectroscopy: a comparison with Raman spectroscopy for analysis and classification of amino acids and dipeptides

Erman K. Oztekin, Sarah E. Smith, David W. Hahn

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Journal of Biomedical Optics, Vol. 20, Issue 4, 047006 (April 2015). https://doi.org/10.1117/1.JBO.20.4.047006

Abstract

Differential-laser induced perturbation spectroscopy (DLIPS) is a new spectral analysis technique for classification and identification, with key potential applications for analysis of complex biomolecular systems. DLIPS takes advantage of the complex ultraviolet (UV) laser–material interactions based on difference spectroscopy by coupling low intensity UV laser perturbation with a traditional spectroscopy probe. Here, we quantify the DLIPS performance using a Raman scattering probe in classification of basic constituents of collagenous tissues, namely, the amino acids glycine,_L-proline, and _L-alanine, and the dipeptides glycine–glycine, glycine–alanine and glycine–proline and compare the performance to a traditional Raman spectroscopy probe via several multivariate analyses. We find that the DLIPS approach yields an ∼40% improvement in discrimination among these tissue building blocks. The effects of the 193-nm perturbation laser are further examined by assessing the photodestruction of targeted material molecular bonds. The DLIPS method with a Raman probe holds promise for future tissue diagnosis, either as a stand-alone technique or as part of an orthogonal biosensing scheme.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Erman K. Oztekin, Sarah E. Smith, and David W. Hahn "Differential laser-induced perturbation Raman spectroscopy: a comparison with Raman spectroscopy for analysis and classification of amino acids and dipeptides," Journal of Biomedical Optics 20(4), 047006 (23 April 2015). https://doi.org/10.1117/1.JBO.20.4.047006

Published: 23 April 2015

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