27 October 2014 Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization
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Abstract
The photoisomerization of relatively nontoxic E-combretastatins to clinically active Z-isomers is shown to occur in solution through both one- and two-photon excitations at 340 and 625 nm, respectively. The photoisomerization is also demonstrated to induce mammalian cell death by a two-photon absorption process at 625 nm. Unlike conventional photodynamic therapy (PDT), the mechanism of photoisomerization is oxygen-independent and active in hypoxic environments such as in tumors. The use of red or near-infrared (NIR) light for two-photon excitation allows greater tissue penetration than conventional UV one-photon excitation. The results provide a baseline for the development of a novel phototherapy that overcomes nondiscriminative systemic toxicity of Z-combretastatins and the limitations of PDT drugs that require the presence of oxygen to promote their activity, with the added benefits of two-photon red or NIR excitation for deeper tissue penetration.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Kathrin M. Scherer, Kathrin M. Scherer, Roger H. Bisby, Roger H. Bisby, Stanley W. Botchway, Stanley W. Botchway, John A. Hadfield, John A. Hadfield, Anthony W. Parker, Anthony W. Parker, } "Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization," Journal of Biomedical Optics 20(5), 051004 (27 October 2014). https://doi.org/10.1117/1.JBO.20.5.051004 . Submission:
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