6 January 2015 Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing
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Low level light therapy (LLLT) has numerous therapeutic benefits, including improving wound healing, but the precise mechanisms involved are not well established; in particular, the underlying role of cytochrome C oxidase (C-ox) as the primary photoacceptor and the associated biochemical mechanisms still require further investigation. We previously showed the nitric oxide (NO) donating drug nitrosyl-cobinamide (NO-Cbi) enhances wound healing through a cGMP/cGMP-dependent protein kinase/ERK1/2 mechanism. Here, we show that the combination of LLLT and NO-Cbi markedly improves wound healing compared to either treatment alone. LLLT-enhanced wound healing proceeded through an electron transport chain-C-ox-dependent mechanism with a reduction of reactive oxygen species and increased adenosine triphosphate production. C-ox was validated as the primary photoacceptor by three observations: increased oxygen consumption, reduced wound healing in the presence of sodium azide, and disassociation of cyanide, a known C-ox ligand, following LLLT. We conclude that LLLT and NO-Cbi accelerate wound healing through two independent mechanisms, the electron transport chain-C-ox pathway and cGMP signaling, respectively, with both resulting in ERK1/2 activation.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Ryan Spitler, Ryan Spitler, Hsiang Ho, Hsiang Ho, Frederique Norpetlian, Frederique Norpetlian, Xiangduo Kong, Xiangduo Kong, Jingjing Jiang, Jingjing Jiang, Kyoko Yokomori, Kyoko Yokomori, Bogi Andersen, Bogi Andersen, Gerry R. Boss, Gerry R. Boss, Michael W. Berns, Michael W. Berns, } "Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing," Journal of Biomedical Optics 20(5), 051022 (6 January 2015). https://doi.org/10.1117/1.JBO.20.5.051022 . Submission:


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