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27 January 2015 Second-harmonic generation scattering directionality predicts tumor cell motility in collagen gels
Kathleen A. Burke, Ryan P. Dawes, Mehar K. Cheema, Amy Van Hove, Danielle S. W. Benoit, Seth W. Perry, Edward Brown
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Journal of Biomedical Optics, Vol. 20, Issue 5, 051024 (January 2015). https://doi.org/10.1117/1.JBO.20.5.051024
Abstract
Second-harmonic generation (SHG) allows for the analysis of tumor collagen structural changes throughout metastatic progression. SHG directionality, measured through the ratio of the forward-propagating to backward-propagating signal (F/B ratio), is affected by collagen fibril diameter, spacing, and disorder of fibril packing within a fiber. As tumors progress, these parameters evolve, producing concurrent changes in F/B. It has been recently shown that the F/B of highly metastatic invasive ductal carcinoma (IDC) breast tumors is significantly different from less metastatic tumors. This suggests a possible relationship between the microstructure of collagen, as measured by the F/B, and the ability of tumor cells to locomote through that collagen. Utilizing in vitro collagen gels of different F/B ratios, we explored the relationship between collagen microstructure and motility of tumor cells in a “clean” environment, free of the myriad cells, and signals found in in vivo. We found a significant relationship between F/B and the total distance traveled by the tumor cell, as well as both the average and maximum velocities of the cells. Consequently, one possible mechanism underlying the observed relationship between tumor F/B and metastatic output in IDC patient samples is a direct influence of collagen structure on tumor cell motility.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Kathleen A. Burke, Ryan P. Dawes, Mehar K. Cheema, Amy Van Hove, Danielle S. W. Benoit, Seth W. Perry, and Edward Brown "Second-harmonic generation scattering directionality predicts tumor cell motility in collagen gels," Journal of Biomedical Optics 20(5), 051024 (27 January 2015). https://doi.org/10.1117/1.JBO.20.5.051024
Published: 27 January 2015
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Cited by 16 scholarly publications.
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KEYWORDS
Tumors
Collagen
Second-harmonic generation
Lymphatic system
Scattering
In vitro testing
Tissues
CHORUS Article. This article was made freely available starting 27 January 2016
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