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2 March 2015 Identification of individual biofilm-forming bacterial cells using Raman tweezers
Ota Samek, Silvie Bernatová, Jan Ježek, Martin Šiler, Mojmir Šerý, Vladislav Krzyzanek, Kamila Hrubanová, Pavel Zemánek, Veronika Holá, Filip Ruzicka
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Journal of Biomedical Optics, Vol. 20, Issue 5, 051038 (March 2015). https://doi.org/10.1117/1.JBO.20.5.051038
Abstract
A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral “Raman fingerprints” obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Ota Samek, Silvie Bernatová, Jan Ježek, Martin Šiler, Mojmir Šerý, Vladislav Krzyzanek, Kamila Hrubanová, Pavel Zemánek, Veronika Holá, and Filip Ruzicka "Identification of individual biofilm-forming bacterial cells using Raman tweezers," Journal of Biomedical Optics 20(5), 051038 (2 March 2015). https://doi.org/10.1117/1.JBO.20.5.051038
Published: 2 March 2015
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Cited by 16 scholarly publications.
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KEYWORDS
Raman spectroscopy
Principal component analysis
Bacteria
Optical tweezers
Imaging spectroscopy
Microorganisms
Proteins
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