Quantitative evaluation of in vivo vital-dye fluorescence endoscopic imaging for the detection of Barrett’s-associated neoplasia

Nadhi Thekkek; Michelle H. Lee; Alexandros D. Polydorides; Daniel G. Rosen; Sharmila Anandasabapathy M.D.; Rebecca Richards-Kortum

doi:10.1117/1.JBO.20.5.056002

7 May 2015 Quantitative evaluation of in vivo vital-dye fluorescence endoscopic imaging for the detection of Barrett’s-associated neoplasia

Nadhi Thekkek, Michelle H. Lee, Alexandros D. Polydorides, Daniel G. Rosen, Sharmila Anandasabapathy M.D., Rebecca Richards-Kortum

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Journal of Biomedical Optics, Vol. 20, Issue 5, 056002 (May 2015). https://doi.org/10.1117/1.JBO.20.5.056002

Abstract

Current imaging tools are associated with inconsistent sensitivity and specificity for detection of Barrett’s-associated neoplasia. Optical imaging has shown promise in improving the classification of neoplasia in vivo. The goal of this pilot study was to evaluate whether in vivo vital dye fluorescence imaging (VFI) has the potential to improve the accuracy of early-detection of Barrett’s-associated neoplasia. In vivo endoscopic VFI images were collected from 65 sites in 14 patients with confirmed Barrett’s esophagus (BE), dysplasia, or esophageal adenocarcinoma using a modular video endoscope and a high-resolution microendoscope (HRME). Qualitative image features were compared to histology; VFI and HRME images show changes in glandular structure associated with neoplastic progression. Quantitative image features in VFI images were identified for objective image classification of metaplasia and neoplasia, and a diagnostic algorithm was developed using leave-one-out cross validation. Three image features extracted from VFI images were used to classify tissue as neoplastic or not with a sensitivity of 87.8% and a specificity of 77.6% (AUC=0.878). A multimodal approach incorporating VFI and HRME imaging can delineate epithelial changes present in Barrett’s-associated neoplasia. Quantitative analysis of VFI images may provide a means for objective interpretation of BE during surveillance.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Nadhi Thekkek, Michelle H. Lee, Alexandros D. Polydorides, Daniel G. Rosen, Sharmila Anandasabapathy M.D., and Rebecca Richards-Kortum "Quantitative evaluation of in vivo vital-dye fluorescence endoscopic imaging for the detection of Barrett’s-associated neoplasia," Journal of Biomedical Optics 20(5), 056002 (7 May 2015). https://doi.org/10.1117/1.JBO.20.5.056002

Published: 7 May 2015

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