22 May 2015 Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy
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We describe rare-earth-doped nanophosphors (RE-NPs) for biological imaging using cathodoluminescence (CL) microscopy based on scanning transmission electron microscopy (STEM). We report the first demonstration of multicolor CL nanobioimaging using STEM with nanophosphors. The CL spectra of the synthesized nanophosphors (Y2O3:Eu, Y2O3:Tb) were sufficiently narrow to be distinguished. From CL images of RE-NPs on an elastic carbon-coated copper grid, the spatial resolution was beyond the diffraction limit of light. Y2O3:Tb and Y2O3:Eu RE-NPs showed a remarkable resistance against electron beam exposure even at high acceleration voltage (80 kV) and retained a CL intensity of more than 97% compared with the initial intensity for 1 min. In biological CL imaging with STEM, heavy-metal-stained cell sections containing the RE-NPs were prepared, and both the CL images of RE-NPs and cellular structures, such as mitochondria, were clearly observed from STEM images with high contrast. The cellular CL imaging using RE-NPs also had high spatial resolution even though heavy-metal-stained cells are normally regarded as highly scattering media. Moreover, since the RE-NPs exhibit photoluminescence (PL) excited by UV light, they are useful for multimodal correlative imaging using CL and PL.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Taichi Furukawa, Taichi Furukawa, Shoichiro Fukushima, Shoichiro Fukushima, Hirohiko Niioka, Hirohiko Niioka, Naoki Yamamoto, Naoki Yamamoto, Jun Miyake, Jun Miyake, Tsutomu Araki, Tsutomu Araki, Mamoru Hashimoto, Mamoru Hashimoto, } "Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy," Journal of Biomedical Optics 20(5), 056007 (22 May 2015). https://doi.org/10.1117/1.JBO.20.5.056007 . Submission:

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