Label-free imaging and quantitative chemical analysis of Alzheimer’s disease brain samples with multimodal multiphoton nonlinear optical microspectroscopy

Jang Hyuk Lee; Dae Hwan Kim; Woo Keun Song; Myoung-Kyu Oh; Do-Kyeong Ko

doi:10.1117/1.JBO.20.5.056013

28 May 2015 Label-free imaging and quantitative chemical analysis of Alzheimer’s disease brain samples with multimodal multiphoton nonlinear optical microspectroscopy

Jang Hyuk Lee, Dae Hwan Kim, Woo Keun Song, Myoung-Kyu Oh, Do-Kyeong Ko

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Journal of Biomedical Optics, Vol. 20, Issue 5, 056013 (May 2015). https://doi.org/10.1117/1.JBO.20.5.056013

Abstract

We developed multimodal multiphoton microspectroscopy using a small-diameter probe with gradient-index lenses and applied it to unstained Alzheimer’s disease (AD) brain samples. Our system maintained the image quality and spatial resolution of images obtained using an objective lens of similar numerical aperture. Multicolor images of AD brain samples were obtained simultaneously by integrating two-photon excited fluorescence and second-harmonic generation on a coherent anti-Stokes Raman scattering (CARS) microendoscope platform. Measurements of two hippocampal regions, the cornus ammonis-1 and dentate gyrus, revealed more lipids, amyloid fibers, and collagen in the AD samples than in the normal samples. Normal and AD brains were clearly distinguished by a large spectral difference and quantitative analysis of the CH mode using CARS microendoscope spectroscopy. We expect this system to be an important diagnosis tool in AD research.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Jang Hyuk Lee, Dae Hwan Kim, Woo Keun Song, Myoung-Kyu Oh, and Do-Kyeong Ko "Label-free imaging and quantitative chemical analysis of Alzheimer’s disease brain samples with multimodal multiphoton nonlinear optical microspectroscopy," Journal of Biomedical Optics 20(5), 056013 (28 May 2015). https://doi.org/10.1117/1.JBO.20.5.056013

Published: 28 May 2015

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