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6 April 2015 Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs
Christopher J. Rowlands, Oliver T. Bruns, Moungi G. Bawendi, Peter T. C. So
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Journal of Biomedical Optics, Vol. 20, Issue 6, 061107 (April 2015). https://doi.org/10.1117/1.JBO.20.6.061107
Abstract
Temporal focusing is a technique for performing axially resolved widefield multiphoton microscopy with a large field of view. Despite significant advantages over conventional point-scanning multiphoton microscopy in terms of imaging speed, the need to collect the whole image simultaneously means that it is expected to achieve a lower penetration depth in common biological samples compared to point-scanning. We assess the penetration depth using a rigorous objective criterion based on the modulation transfer function, comparing it to point-scanning multiphoton microscopy. Measurements are performed in a variety of mouse organs in order to provide practical guidance as to the achievable penetration depth for both imaging techniques. It is found that two-photon scanning microscopy has approximately twice the penetration depth of temporal-focusing microscopy, and that penetration depth is organ-specific; the heart has the lowest penetration depth, followed by the liver, lungs, and kidneys, then the spleen, and finally white adipose tissue.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Christopher J. Rowlands, Oliver T. Bruns, Moungi G. Bawendi, and Peter T. C. So "Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs," Journal of Biomedical Optics 20(6), 061107 (6 April 2015). https://doi.org/10.1117/1.JBO.20.6.061107
Published: 6 April 2015
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Cited by 10 scholarly publications.
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KEYWORDS
Spatial frequencies
Microscopy
Modulation transfer functions
Tissues
Two photon excitation microscopy
Microscopes
Multiphoton microscopy
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