25 June 2015 Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics
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Intravital microscopy (IVM) is a well-established imaging technique for real-time monitoring of microscale lung tissue dynamics. Although accepted as a gold standard in respiratory research, its characteristic image features are scarcely understood, especially when trying to determine the actual position of alveolar walls. To allow correct interpretation of these images with respect to the true geometry of the lung parenchyma, we analyzed IVM data of alveoli in a mouse model in comparison with simultaneously acquired optical coherence tomography images. Several IVM characteristics, such as double ring structures or disappearing alveoli in regions of liquid filling, could be identified and related to the position of alveoli relative to each other. Utilizing a ray tracing approach based on an idealized geometry of the mouse lung parenchyma, two major reflection processes could be attributed to the IVM image formation: partial reflection and total internal reflection between adjacent alveoli. Considering the origin of the reflexes, a model was developed to determine the true position of alveolar walls within IVM images. These results allow thorough understanding of IVM data and may serve as a basis for the correction of alveolar sizes for more accurate quantitative analysis within future studies of lung tissue dynamics.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Maria Gaertner, Maria Gaertner, Kerstin Schirrmann, Kerstin Schirrmann, Christian Schnabel, Christian Schnabel, Sven Meissner, Sven Meissner, Ulrich Kertzscher, Ulrich Kertzscher, Lars Kirsten, Lars Kirsten, Edmund Koch, Edmund Koch, } "Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics," Journal of Biomedical Optics 20(6), 066009 (25 June 2015). https://doi.org/10.1117/1.JBO.20.6.066009 . Submission:

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