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11 August 2015 Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer
Adi Sheinfeld, Will J. Eldridge, Adam Wax
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Journal of Biomedical Optics, Vol. 20, Issue 8, 086002 (August 2015). https://doi.org/10.1117/1.JBO.20.8.086002
Abstract
We present a dual-modality system for both structural and molecular cell imaging based on coregistered quantitative phase imaging (QPI) and photoacoustic microscopy (PAM). The QPI system was based on off-axis holography, whereas the PAM system comprised a sinusoidally modulated optical source for excitation and a narrow-band low profile and low-cost ring ultrasonic transducer for detection. This approach facilitated a simple confocal alignment of the excitation beams of both modalities and the ultrasonic detector. This system was demonstrated by imaging endogenous molecules in red blood cells (RBCs) as well as by imaging exogenous molecular labels on cancer cells using gold nanoparticles (GNPs) functionalized to target epidermal growth factor receptor. QPI provided high resolution imaging of the cellular structures while PAM provided molecular contrast. This dual-modality microscopy method can potentially be implemented as a compact and low cost cellular diagnostic assay.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Adi Sheinfeld, Will J. Eldridge, and Adam Wax "Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer," Journal of Biomedical Optics 20(8), 086002 (11 August 2015). https://doi.org/10.1117/1.JBO.20.8.086002
Published: 11 August 2015
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KEYWORDS
Transducers
Imaging systems
Signal to noise ratio
Phase imaging
Ultrasonics
Ferroelectric materials
Modulation
CHORUS Article. This article was made freely available starting 10 August 2016
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