21 September 2015 Terahertz spectroscopy of oligonucleotides in aqueous solutions
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A terahertz (THz) spectroscopic study is carried out to analyze DNA mutations in a label-free manner. Three newly designed liquid sample cells are considered and the best is selected as the sample carrier for THz transmission spectroscopic analyses. Discrimination based on spectral signatures of single-base mutations on single-stranded 20 nt oligonucleotides has been shown possible experimentally. The results clearly attest the ability of this promising approach for label-free analyses of single-base mutations of DNA molecules. This study has demonstrated that the THz spectroscopic technology can be considered as a potential diagnostic tool for investigating molecular reactions, such as DNA mutations.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Mingjie Tang, Mingjie Tang, Qing Huang, Qing Huang, Dongshan Wei, Dongshan Wei, Guozhong Zhao, Guozhong Zhao, Tianying Chang, Tianying Chang, Kuan Kou, Kuan Kou, Min Wang, Min Wang, Chunlei Du, Chunlei Du, Wei-ling Fu, Wei-ling Fu, Hong-Liang Cui, Hong-Liang Cui, } "Terahertz spectroscopy of oligonucleotides in aqueous solutions," Journal of Biomedical Optics 20(9), 095009 (21 September 2015). https://doi.org/10.1117/1.JBO.20.9.095009 . Submission:

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