10 September 2015 Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors
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J. of Biomedical Optics, 20(9), 096006 (2015). doi:10.1117/1.JBO.20.9.096006
Photoacoustic imaging (PAI) contributes to tumor diagnosis through the use of PAI probes that effectively accumulate in tumors. Previously, we developed a symmetrical cyanine dye, IC7-1-Bu, which showed high potential as a PAI probe because of its high tumor targeting ability and sufficient in vivo PA signal. However, IC7-1-Bu lacks photostability for multiple laser irradiations, so we developed stabilized PAI probes using IC7-1-Bu as a lead compound. We focused on the effect of singlet oxygen (1O2) generated by excited PAI probes on probe degeneration. We introduced a triplet-state quencher (TSQ) moiety into IC7-1-Bu to quench 1O2 generation and designed three IC-n-T derivatives with different linker lengths (n indicates linker length). The IC-n-T derivatives emitted in vitro PA signals that were comparable to IC7-1-Bu and significantly reduced 1O2 generation while showing improved photostability against multiple irradiations. Of the three derivatives evaluated, IC-5-T accumulated in tumors effectively to allow clear PAI of tumors in vivo. Furthermore, the photostability of IC-5-T was 1.5-fold higher than that of IC7-1-Bu in in vivo sequential PAI. These results suggest that IC-5-T is a potential PAI probe for in vivo sequential tumor imaging.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Satoru Onoe, Takashi Temma, Kengo Kanazaki, Masahiro Ono, Hideo Saji, "Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors," Journal of Biomedical Optics 20(9), 096006 (10 September 2015). https://doi.org/10.1117/1.JBO.20.9.096006

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