10 September 2015 Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors
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Abstract
Photoacoustic imaging (PAI) contributes to tumor diagnosis through the use of PAI probes that effectively accumulate in tumors. Previously, we developed a symmetrical cyanine dye, IC7-1-Bu, which showed high potential as a PAI probe because of its high tumor targeting ability and sufficient in vivo PA signal. However, IC7-1-Bu lacks photostability for multiple laser irradiations, so we developed stabilized PAI probes using IC7-1-Bu as a lead compound. We focused on the effect of singlet oxygen (1O2) generated by excited PAI probes on probe degeneration. We introduced a triplet-state quencher (TSQ) moiety into IC7-1-Bu to quench 1O2 generation and designed three IC-n-T derivatives with different linker lengths (n indicates linker length). The IC-n-T derivatives emitted in vitro PA signals that were comparable to IC7-1-Bu and significantly reduced 1O2 generation while showing improved photostability against multiple irradiations. Of the three derivatives evaluated, IC-5-T accumulated in tumors effectively to allow clear PAI of tumors in vivo. Furthermore, the photostability of IC-5-T was 1.5-fold higher than that of IC7-1-Bu in in vivo sequential PAI. These results suggest that IC-5-T is a potential PAI probe for in vivo sequential tumor imaging.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Satoru Onoe, Satoru Onoe, Takashi Temma, Takashi Temma, Kengo Kanazaki, Kengo Kanazaki, Masahiro Ono, Masahiro Ono, Hideo Saji, Hideo Saji, } "Development of photostabilized asymmetrical cyanine dyes for in vivo photoacoustic imaging of tumors," Journal of Biomedical Optics 20(9), 096006 (10 September 2015). https://doi.org/10.1117/1.JBO.20.9.096006 . Submission:
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