26 January 2016 Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy
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J. of Biomedical Optics, 21(1), 016011 (2016). doi:10.1117/1.JBO.21.1.016011
Abstract
We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M(2)) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μd) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Chukwuemeka Okoro, Kimani C. Toussaint, "Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy," Journal of Biomedical Optics 21(1), 016011 (26 January 2016). http://dx.doi.org/10.1117/1.JBO.21.1.016011
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KEYWORDS
Second-harmonic generation

Polarization

Microscopy

Tissues

Mueller matrices

Collagen

Multiphoton microscopy

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