18 January 2016 Intraoperative optical assessment of photodynamic therapy response of superficial oral squamous cell carcinoma
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Abstract
This study investigated whether diffuse optical spectroscopy (DOS) measurements could assess clinical response to photodynamic therapy (PDT) in patients with head and neck squamous cell carcinoma (HNSCC). In addition, the correlation between parameters measured with DOS and the crosslinking of signal transducer and activator of transcription 3 (STAT3), a molecular marker for PDT-induced photoreaction, was investigated. Thirteen patients with early stage HNSCC received the photosensitizer 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH) and DOS measurements were performed before and after PDT in the operating room (OR). In addition, biopsies were acquired after PDT to assess the STAT3 crosslinking. Parameters measured with DOS, including blood volume fraction, blood oxygen saturation (StO2), HPPH concentration (cHPPH), HPPH fluorescence, and blood flow index (BFI), were compared to the pathologic response and the STAT3 crosslinking. The best individual predictor of pathological response was a change in cHPPH (sensitivity=60%, specificity=100%), while discrimination analysis using a two-parameter classifier (change in cHPPH and change in StO2) classified pathological response with 100% sensitivity and 100% specificity. BFI showed the best correlation with the crosslinking of STAT3. These results indicate that DOS-derived parameters can assess the clinical response in the OR, allowing for earlier reintervention if needed.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Daniel J. Rohrbach, Daniel J. Rohrbach, Nestor R. Rigual, Nestor R. Rigual, Hassan Arshad, Hassan Arshad, Gal Shafirstein, Gal Shafirstein, Erin C. Tracy, Erin C. Tracy, Gregory Wilding, Gregory Wilding, Mihai Merzianu, Mihai Merzianu, Michele T. Cooper, Michele T. Cooper, Heinz Baumann, Heinz Baumann, Barbara W. Henderson, Barbara W. Henderson, Ulas Sunar, Ulas Sunar, } "Intraoperative optical assessment of photodynamic therapy response of superficial oral squamous cell carcinoma," Journal of Biomedical Optics 21(1), 018002 (18 January 2016). https://doi.org/10.1117/1.JBO.21.1.018002 . Submission: Received: 11 September 2015; Accepted: 11 December 2015
Received: 11 September 2015; Accepted: 11 December 2015; Published: 18 January 2016
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