7 October 2016 Evaluation of the diagnostic potential of ex vivo Raman spectroscopy in gastric cancers: fingerprint versus high wavenumber
Author Affiliations +
Abstract
The aim of this study was to apply Raman spectroscopy in the high wavenumber (HW) region (2800 to 3000  cm−1) for ex vivo detection of gastric cancer and compare its diagnostic potential with that of the fingerprint (FP) region (800 to 1800  cm−1). Raman spectra were collected in the FP and HW regions to differentiate between normal mucosa (n=38) and gastric cancer (n=37). The distinctive Raman spectral differences between normal and cancer tissues are observed at 853, 879, 1157, 1319, 1338, 1448, and 2932  cm−1 and are primarily related to proteins, lipids, nucleic acids, collagen, and carotenoids in the tissue. In FP and HW Raman spectroscopy for diagnosis of gastric cancer, multivariate diagnostic algorithms based on partial-least-squares discriminant analysis, together with leave-one-sample-out cross validation, yielded diagnostic sensitivities of 94.59% and 81.08%, and specificities of 86.84% and 71.05%, respectively. Receiver operating characteristic analysis further confirmed that the FP region model performance is superior to that of the HW region model. Better differentiation between normal and gastric cancer tissues can be achieved using FP Raman spectroscopy and PLS-DA techniques, but the complementary natures of the FP and HW regions make both of them useful in diagnosis of gastric cancer.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xueqian Zhou, Jianhua Dai, Yao Chen, Guangjie Duan, Yulong Liu, Hua Zhang, Hongbo Wu, Guiyong Peng, "Evaluation of the diagnostic potential of ex vivo Raman spectroscopy in gastric cancers: fingerprint versus high wavenumber," Journal of Biomedical Optics 21(10), 105002 (7 October 2016). https://doi.org/10.1117/1.JBO.21.10.105002 . Submission:
JOURNAL ARTICLE
7 PAGES


SHARE
Back to Top