4 November 2016 Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry
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Abstract
Abnormal blood flow during early cardiovascular development has been identified as a key factor in the pathogenesis of congenital heart disease; however, the mechanisms by which altered hemodynamics induce cardiac malformations are poorly understood. This study used outflow tract (OFT) banding to model increased afterload, pressure, and blood flow velocities at tubular stages of heart development and characterized the immediate changes in cardiac wall motion due to banding in chicken embryo models with light microscopy-based video densitometry. Optical videos were used to acquire two-dimensional heart image sequences over the cardiac cycle, from which intensity data were extracted along the heart centerline at several locations in the heart ventricle and OFT. While no changes were observed in the synchronous contraction of the ventricle with banding, the peristaltic-like wall motion in the OFT was significantly affected. Our data provide valuable insight into early cardiac biomechanics and its characterization using a simple light microscopy-based imaging modality.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Stephanie Stovall, Stephanie Stovall, Madeline Midgett, Madeline Midgett, Kent Thornburg, Kent Thornburg, Sandra Rugonyi, Sandra Rugonyi, } "Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry," Journal of Biomedical Optics 21(11), 116003 (4 November 2016). https://doi.org/10.1117/1.JBO.21.11.116003 . Submission:
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