8 November 2016 Imaging technique for real-time temperature monitoring during cryotherapy of lesions
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J. of Biomedical Optics, 21(11), 116007 (2016). doi:10.1117/1.JBO.21.11.116007
Noninvasive real-time temperature imaging during thermal therapies is able to significantly improve clinical outcomes. An optoacoustic (OA) temperature monitoring method is proposed for noninvasive real-time thermometry of vascularized tissue during cryotherapy. The universal temperature-dependent optoacoustic response (ThOR) of red blood cells (RBCs) is employed to convert reconstructed OA images to temperature maps. To obtain the temperature calibration curve for intensity-normalized OA images, we measured ThOR of 10 porcine blood samples in the range of temperatures from 40°C to −16°C and analyzed the data for single measurement variations. The nonlinearity (ΔTmax) and the temperature of zero OA response (T0) of the calibration curve were found equal to 11.4±0.1°C and −13.8±0.1°C, respectively. The morphology of RBCs was examined before and after the data collection confirming cellular integrity and intracellular compartmentalization of hemoglobin. For temperatures below 0°C, which are of particular interest for cryotherapy, the accuracy of a single temperature measurement was ±1°C, which is consistent with the clinical requirements. Validation of the proposed OA temperature imaging technique was performed for slow and fast cooling of blood samples embedded in tissue-mimicking phantoms.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Elena Petrova, Anton Liopo, Vyacheslav Nadvoretskiy, Sergey Ermilov, "Imaging technique for real-time temperature monitoring during cryotherapy of lesions," Journal of Biomedical Optics 21(11), 116007 (8 November 2016). https://doi.org/10.1117/1.JBO.21.11.116007


Temperature metrology




Tissue optics

Real time imaging

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