10 December 2016 Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography
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Abstract
We performed the feasibility study using speckle variance optical coherence tomography (SvOCT) to monitor the thermally induced protein denaturation and coagulation process as a function of temperature and depth. SvOCT provided the depth-resolved image of protein denaturation and coagulation with microscale resolution. This study was conducted using egg white. During the heating process, as the temperature increased, increases in the speckle variance signal was observed as the egg white proteins coagulated. Additionally, by calculating the cross-correlation coefficient in specific areas, denaturized egg white conditions were successfully estimated. These results indicate that SvOCT could be used to monitor the denaturation process of various proteins.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Changho Lee, Gyeongwoo Cheon, Do-Hyun Kim, Jin U. Kang, "Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography," Journal of Biomedical Optics 21(12), 125004 (10 December 2016). https://doi.org/10.1117/1.JBO.21.12.125004 . Submission:
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