Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats

Laura Di Sieno; Georges Bettega; Michel Berger; Cynthia Hamou; Marion Aribert; Alberto Dalla Mora; Agathe Puszka; Henri Grateau; Davide Contini; Lionel Hervé; Jean-Luc Coll; Jean-Marc Dinten; Antonio Pifferi; Anne Planat-Chrétien

doi:10.1117/1.JBO.21.2.025004

2 February 2016 Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats

Laura Di Sieno, Georges Bettega, Michel Berger, Cynthia Hamou, Marion Aribert, Alberto Dalla Mora, Agathe Puszka, Henri Grateau, Davide Contini, Lionel Hervé, Jean-Luc Coll, Jean-Marc Dinten, Antonio Pifferi, Anne Planat-Chrétien

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Journal of Biomedical Optics, Vol. 21, Issue 2, 025004 (February 2016). https://doi.org/10.1117/1.JBO.21.2.025004

Abstract

The noninvasive assessment of flap viability in autologous reconstruction surgery is still an unmet clinical need. To cope with this problem, we developed a proof-of-principle fully automatized setup for fast time-gated diffuse optical tomography exploiting Mellin–Laplace transform to obtain three-dimensional tomographic reconstructions of oxy- and deoxy-hemoglobin concentrations. We applied this method to perform preclinical tests on rats inducing total venous occlusion in the cutaneous abdominal flaps. Notwithstanding the use of just four source-detector couples, we could detect a spatially localized increase of deoxyhemoglobin following the occlusion (up to 550 μM in 54 min). Such capability to image spatio-temporal evolution of blood perfusion is a key issue for the noninvasive monitoring of flap viability.

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Laura Di Sieno, Georges Bettega, Michel Berger, Cynthia Hamou, Marion Aribert, Alberto Dalla Mora, Agathe Puszka, Henri Grateau, Davide Contini, Lionel Hervé, Jean-Luc Coll, Jean-Marc Dinten, Antonio Pifferi, and Anne Planat-Chrétien "Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats," Journal of Biomedical Optics 21(2), 025004 (2 February 2016). https://doi.org/10.1117/1.JBO.21.2.025004

Published: 2 February 2016

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