17 February 2016 Simultaneous blood flow and blood oxygenation measurements using a combination of diffuse speckle contrast analysis and near-infrared spectroscopy
Author Affiliations +
Abstract
A combined diffuse speckle contrast analysis (DSCA)–near-infrared spectroscopy (NIRS) system is proposed to simultaneously measure qualitative blood flow and blood oxygenation changes in human tissue. The system employs an optical switch to alternate two laser sources at two different wavelengths and a CCD camera to capture the speckle image. Therefore, an optical density can be measured from two wavelengths for NIRS measurements and a speckle contrast can be calculated for DSCA measurements. In order to validate the system, a flow phantom test and an arm occlusion protocol for arterial and venous occlusion were performed. Shorter exposure times (<1  ms) show a higher drop (between 50% and 66%) and recovery of 1/KS2 values after occlusion (approximately 150%), but longer exposure time (3 ms) shows more consistent hemodynamic changes. For four subjects, the 1/KS2 values dropped to an average of 82.1±4.0% during the occlusion period and the average recovery of 1/KS2 values after occlusion was 109.1±0.8%. There was also an approximately equivalent amplitude change in oxyhemoglobin (OHb) and deoxyhemoglobin (RHb) during arterial occlusion (max RHb=0.0085±0.0024  mM/DPF, min OHb=−0.0057±0.0044  mM/DPF). The sensitivity of the system makes it a suitable modality to observe qualitative hemodynamic trends during induced physiological changes.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Myeongsu Seong, Zephaniah Phillips, Phuong Minh Mai, Chaebeom Yeo, Cheol Song, Kijoon Lee, Jae Gwan Kim, "Simultaneous blood flow and blood oxygenation measurements using a combination of diffuse speckle contrast analysis and near-infrared spectroscopy," Journal of Biomedical Optics 21(2), 027001 (17 February 2016). https://doi.org/10.1117/1.JBO.21.2.027001 . Submission:
JOURNAL ARTICLE
5 PAGES


SHARE
Back to Top