8 March 2016 Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope
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Abstract
Five dimensional microscopy with a 12-fs laser scanning microscope based on spectrally resolved two-photon autofluorescence lifetime and second-harmonic generation (SHG) imaging was used to characterize all layers of the porcine cornea. This setup allowed the simultaneous excitation of both metabolic cofactors, NAD(P)H and flavins, and their discrimination based on their spectral emission properties and fluorescence decay characteristics. Furthermore, the architecture of the stromal collagen fibrils was assessed by SHG imaging in both forward and backward directions. Information on the metabolic state and the tissue architecture of the porcine cornea were obtained with subcellular resolution, and high temporal and spectral resolutions.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Ana Batista, Ana Batista, Hans Georg Breunig, Hans Georg Breunig, Aisada Uchugonova, Aisada Uchugonova, António Miguel Morgado, António Miguel Morgado, Karsten König, Karsten König, } "Two-photon spectral fluorescence lifetime and second-harmonic generation imaging of the porcine cornea with a 12-femtosecond laser microscope," Journal of Biomedical Optics 21(3), 036002 (8 March 2016). https://doi.org/10.1117/1.JBO.21.3.036002 . Submission:
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