11 March 2016 Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain
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Abstract
We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50  μm deep inside living Drosophila brain.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Mélanie Pedrazzani, Vincent Loriette, Paul Tchenio, Sakina Benrezzak, Daniele Nutarelli, Alexandra Fragola, "Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain," Journal of Biomedical Optics 21(3), 036006 (11 March 2016). https://doi.org/10.1117/1.JBO.21.3.036006 . Submission:
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