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25 April 2016 Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons
Simon Labrecque, Jean-Philippe Sylvestre, Stephane Marcet, Francesca Mangiarini, Brice Bourgoin, Marc Verhaegen, Sébastien Blais-Ouellette, Paul De Koninck
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Journal of Biomedical Optics, Vol. 21, Issue 4, 046008 (April 2016). https://doi.org/10.1117/1.JBO.21.4.046008
Abstract
The efficacy of existing therapies and the discovery of innovative treatments for central nervous system (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. To improve our capability to investigate complex mechanisms of synaptic signaling and remodeling, we designed a fluorescence hyperspectral imaging platform to simultaneously track different subtypes of individual neurotransmitter receptors trafficking in and out of synapses. This imaging platform allows simultaneous image acquisition of at least five fluorescent markers in living neurons with a high-spatial resolution. We used quantum dots emitting at different wavelengths and functionalized to specifically bind to single receptors on the membrane of living neurons. The hyperspectral imaging platform enabled the simultaneous optical tracking of five different synaptic proteins, including subtypes of glutamate receptors (mGluR and AMPAR) and postsynaptic signaling proteins. It also permitted the quantification of their mobility after treatments with various pharmacological agents. This technique provides an efficient method to monitor several synaptic proteins at the same time, which could accelerate the screening of effective compounds for treatment of CNS disorders.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Simon Labrecque, Jean-Philippe Sylvestre, Stephane Marcet, Francesca Mangiarini, Brice Bourgoin, Marc Verhaegen, Sébastien Blais-Ouellette, and Paul De Koninck "Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons," Journal of Biomedical Optics 21(4), 046008 (25 April 2016). https://doi.org/10.1117/1.JBO.21.4.046008
Published: 25 April 2016
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Cited by 9 scholarly publications.
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KEYWORDS
Receptors
Neurons
Imaging systems
Cameras
Hyperspectral imaging
Proteins
Luminescence
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