1 June 2016 Cell optoporation with a sub-15 fs and a 250-fs laser
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Abstract
We employed two commercially available femtosecond lasers, a Ti:sapphire and a ytterbium-based oscillator, to directly compare from a user’s practical point-of-view in one common experimental setup the efficiencies of transient laser-induced cell membrane permeabilization, i.e., of so-called optoporation. The experimental setup consisted of a modified multiphoton laser-scanning microscope employing high-NA focusing optics. An automatic cell irradiation procedure was realized with custom-made software that identified cell positions and controlled relevant hardware components. The Ti:sapphire and ytterbium-based oscillators generated broadband sub-15-fs pulses around 800 nm and 250-fs pulses at 1044 nm, respectively. A higher optoporation rate and posttreatment viability were observed for the shorter fs pulses, confirming the importance of multiphoton effects for efficient optoporation.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Hans Georg Breunig, Hans Georg Breunig, Ana Batista, Ana Batista, Aisada Uchugonova, Aisada Uchugonova, Karsten König, Karsten König, } "Cell optoporation with a sub-15 fs and a 250-fs laser," Journal of Biomedical Optics 21(6), 060501 (1 June 2016). https://doi.org/10.1117/1.JBO.21.6.060501 . Submission:
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