9 June 2016 ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems
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Abstract
We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Veronica Sorgato, Veronica Sorgato, Michel Berger, Michel Berger, Charlotte Emain, Charlotte Emain, Christine Vever-Bizet, Christine Vever-Bizet, Jean-Marc Dinten, Jean-Marc Dinten, Geneviève Bourg-Heckly, Geneviève Bourg-Heckly, Anne Planat-Chrétien, Anne Planat-Chrétien, } "ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems," Journal of Biomedical Optics 21(6), 065003 (9 June 2016). https://doi.org/10.1117/1.JBO.21.6.065003 . Submission:
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