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10 May 2016 Polarized light imaging specifies the anisotropy of light scattering in the superficial layer of a tissue
Steven L. Jacques, Stéphane Roussel, Ravikant Venkata Samatham
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Journal of Biomedical Optics, Vol. 21, Issue 7, 071115 (May 2016). https://doi.org/10.1117/1.JBO.21.7.071115
Abstract
This report describes how optical images acquired using linearly polarized light can specify the anisotropy of scattering (g) and the ratio of reduced scattering [μ's=μs(1−g)] to absorption (μa), i.e., N′=μ's/μa. A camera acquired copolarized (HH) and crosspolarized (HV) reflectance images of a tissue (skin), which yielded images based on the intensity ( I=HH+HV) and difference (Q=HH−HV) of reflectance images. Monte Carlo simulations generated an analysis grid (or lookup table), which mapped Q and I into a grid of g versus N′, i.e., g(Q,I) and N′(Q,I). The anisotropy g is interesting because it is sensitive to the submicrometer structure of biological tissues. Hence, polarized light imaging can monitor shifts in the submicrometer (50 to 1000 nm) structure of tissues. The Q values for forearm skin on two subjects (one Caucasian, one pigmented) were in the range of 0.046±0.007 (24), which is the mean±SD for 24 measurements on 8 skin sites×3 visible wavelengths, 470, 524, and 625 nm, which indicated g values of 0.67±0.07 (24).
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Steven L. Jacques, Stéphane Roussel, and Ravikant Venkata Samatham "Polarized light imaging specifies the anisotropy of light scattering in the superficial layer of a tissue," Journal of Biomedical Optics 21(7), 071115 (10 May 2016). https://doi.org/10.1117/1.JBO.21.7.071115
Published: 10 May 2016
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Cited by 20 scholarly publications and 1 patent.
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KEYWORDS
Skin
Light scattering
Tissues
Anisotropy
Tissue optics
Scattering
Optical spheres
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