19 July 2016 Damage induced in red blood cells by infrared optical trapping: an evaluation based on elasticity measurements
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Abstract
We evaluated the damage caused to optically trapped red blood cells (RBCs) after 1 or 2 min of exposure to near-infrared (NIR) laser beams at 785 or 1064 nm. Damage was quantified by measuring cell elasticity using an automatic, real-time, homemade, optical tweezer system. The measurements, performed on a significant number (hundreds) of cells, revealed an overall deformability decrease up to ∼104% after 2 min of light exposure, under 10 mW optical trapping for the 785-nm wavelength. Wavelength dependence of the optical damage is attributed to the light absorption by hemoglobin. The results provided evidence that RBCs have their biomechanical properties affected by NIR radiation. Our findings establish limits for laser applications with RBCs.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Marcos A. S. de Oliveira, Diógenes S. Moura, Adriana Fontes, Renato E. de Araujo, "Damage induced in red blood cells by infrared optical trapping: an evaluation based on elasticity measurements," Journal of Biomedical Optics 21(7), 075012 (19 July 2016). https://doi.org/10.1117/1.JBO.21.7.075012 . Submission:
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