Quantitative index imaging of coculture cells by scanning focused refractive index microscopy

Teng-Qian Sun; Qing Ye; Fen Hu; Shi-ke Liu; Xiao-Wan Wang; Jin Wang; Zhi-Chao Deng; Jian-Chun Mei; Wen-Yuan Zhou; Chun-Ping Zhang; Xin-Yu Wang; Lei-Ting Pan; Jian-Guo Tian

doi:10.1117/1.JBO.21.8.086016

26 August 2016 Quantitative index imaging of coculture cells by scanning focused refractive index microscopy

Teng-Qian Sun, Qing Ye, Fen Hu, Shi-ke Liu, Xiao-Wan Wang, Jin Wang, Zhi-Chao Deng, Jian-Chun Mei, Wen-Yuan Zhou, Chun-Ping Zhang, Xin-Yu Wang, Lei-Ting Pan, Jian-Guo Tian

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Journal of Biomedical Optics, Vol. 21, Issue 8, 086016 (August 2016). https://doi.org/10.1117/1.JBO.21.8.086016

Abstract

We report the quantitative refractive index (RI) imaging of cocultured cells in their living environment by scanning focused refractive index microscopy (SFRIM). Mouse microglial cells and synovial cells are cocultured on the top surface of a trapezoid prism. The RI imaging of living cells is obtained in a reflection-type method. The RI information is deduced with the simple derivative total internal reflection method, where a complex retrieval algorithm or reconstruction process is unnecessary. The outline of each cell is determined according to the RI value compared with that of the immersion liquid. The cocultured cells can be discriminated in the RI image. The measurement is nondestructive and label-free. The experimental results prove that SFRIM is a promising tool in the field of biological optics.

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Teng-Qian Sun, Qing Ye, Fen Hu, Shi-ke Liu, Xiao-Wan Wang, Jin Wang, Zhi-Chao Deng, Jian-Chun Mei, Wen-Yuan Zhou, Chun-Ping Zhang, Xin-Yu Wang, Lei-Ting Pan, and Jian-Guo Tian "Quantitative index imaging of coculture cells by scanning focused refractive index microscopy," Journal of Biomedical Optics 21(8), 086016 (26 August 2016). https://doi.org/10.1117/1.JBO.21.8.086016

Published: 26 August 2016

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