19 August 2016 Low-level laser therapy induces an upregulation of collagen gene expression during the initial process of bone healing: a microarray analysis
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Abstract
This study investigates the histological modifications produced by low level laser therapy (LLLT) on the first day of bone repair, as well as evaluates the LLLT effects on collagen expression on the site of a fracture. Twenty Wistar rats were distributed into a control group (CG) and a laser group (LG). Laser irradiation of Ga-Al-As laser 830 nm, 30 mW, 94 s, 2.8 J was performed in five sessions. Animals were euthanized on day 5 postsurgery. Histopathological analysis showed that LLLT was able to increase deposition of granulation tissue and newly formed bone at the site of the injury. In addition, picrosirius analysis showed that collagen fiber organization in the LG was enhanced compared to CG. Microarray analysis demonstrated that LLLT produced an upregulation type I collagen (COL-I). Immunohistochemical analysis revealed that the subjects that were treated presented a higher immunoexpression of COL-I. Our findings indicated that LLLT improves bone healing by producing a significant increase in the expression of collagen genes.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Carla Roberta Tim, Carla Roberta Tim, Paulo Sérgio Bossini, Paulo Sérgio Bossini, Hueliton Wilian Kido, Hueliton Wilian Kido, Iran Malavazi, Iran Malavazi, Marcia Regina von Zeska Kress, Marcia Regina von Zeska Kress, Marcelo Falsarella Carazzolle, Marcelo Falsarella Carazzolle, Ana Cláudia Rennó, Ana Cláudia Rennó, Nivaldo Antonio Parizotto, Nivaldo Antonio Parizotto, } "Low-level laser therapy induces an upregulation of collagen gene expression during the initial process of bone healing: a microarray analysis," Journal of Biomedical Optics 21(8), 088001 (19 August 2016). https://doi.org/10.1117/1.JBO.21.8.088001 . Submission:
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