6 September 2016 Integrated quantitative phase and birefringence microscopy for imaging malaria-infected red blood cells
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Abstract
A dual-modality birefringence/phase imaging system is presented. The system features a crystal retarder that provides polarization mixing and generates two interferometric carrier waves in a single signal spectrum. The retardation and orientation of sample birefringence can then be measured simultaneously based on spectral multiplexing interferometry. Further, with the addition of a Nomarski prism, the same setup can be used for quantitative differential interference contrast (DIC) imaging. Sample phase can then be obtained with two-dimensional integration. In addition, birefringence-induced phase error can be corrected using the birefringence data. This dual-modality approach is analyzed theoretically with Jones calculus and validated experimentally with malaria-infected red blood cells. The system generates not only corrected DIC and phase images, but a birefringence map that highlights the distribution of hemozoin crystals.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Chengshuai Li, Chengshuai Li, Shichao Chen, Shichao Chen, Michael Klemba, Michael Klemba, Yizheng Zhu, Yizheng Zhu, } "Integrated quantitative phase and birefringence microscopy for imaging malaria-infected red blood cells," Journal of Biomedical Optics 21(9), 090501 (6 September 2016). https://doi.org/10.1117/1.JBO.21.9.090501 . Submission:
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