21 September 2016 Profiling of individual human red blood cells under osmotic stress using defocusing microscopy
Author Affiliations +
Abstract
We use a quantitative phase imaging technique, defocusing microscopy (DM), to measure morphological, chemical, and mechanical parameters of individual red blood cells (RBCs) immersed in solutions with different osmolalities. We monitor the RBCs’ radius, volume, surface area, sphericity index, and hemoglobin content and concentration. The complete shape of cells is recovered and the effects of their adhesion to the glass substrate are observed. Finally, membrane fluctuation measurements give us information about the cells deformability.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Paula Magda S. Roma, Paula Magda S. Roma, Livia Siman, Livia Siman, Barbara Hissa, Barbara Hissa, Ubirajara Agero, Ubirajara Agero, Erika M. Braga, Erika M. Braga, Oscar N. Mesquita, Oscar N. Mesquita, } "Profiling of individual human red blood cells under osmotic stress using defocusing microscopy," Journal of Biomedical Optics 21(9), 090505 (21 September 2016). https://doi.org/10.1117/1.JBO.21.9.090505 . Submission:
JOURNAL ARTICLE
4 PAGES


SHARE
Back to Top