16 September 2016 Poly(3-hydroxybutyrate) anabolism in Cupriavidus necator cultivated at various carbon-to-nitrogen ratios: insights from single-cell Raman spectroscopy
Author Affiliations +
Abstract
Cupriavidus necator accumulates large amounts of poly(3-hydroxybutyrate) (PHB), a biodegradable substitute for petroleum-based plastics, under certain nutrient conditions. Conventional solvent-extraction-based methods for PHB quantification only obtain average information from cell populations and, thus, mask the heterogeneity among individual cells. Laser tweezers Raman spectroscopy (LTRS) was used to monitor dynamic changes in the contents of PHB, nucleic acids, and proteins in
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
Zhanhua Tao, Zhanhua Tao, Pengfei Zhang, Pengfei Zhang, Zhaojun Qin, Zhaojun Qin, Yong-Qing Li, Yong-Qing Li, Guiwen Wang, Guiwen Wang, } "Poly(3-hydroxybutyrate) anabolism in Cupriavidus necator cultivated at various carbon-to-nitrogen ratios: insights from single-cell Raman spectroscopy," Journal of Biomedical Optics 21(9), 097005 (16 September 2016). https://doi.org/10.1117/1.JBO.21.9.097005 . Submission:
JOURNAL ARTICLE
7 PAGES


SHARE
Back to Top