20 October 2017 Changes in hemoglobin–oxygen affinity with shape variations of red blood cells
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Abstract
Shape variations of red blood cells (RBCs) are known to occur upon exposure to various drugs or under diseased conditions. The commonly observed discocytic RBCs can be transformed to echinocytic or stomatocytic shape under such conditions. Raman spectra of the three major shape variations, namely discocyte, echinocyte, and stomatocyte, of RBCs were studied while subjecting the cells to oxygenated and deoxygenated conditions. Analysis of the recorded spectra suggests an increased level of hemoglobin (Hb)–oxygen affinity for the echinocytes. Also, some level of Hb degradation could be noticed for the deoxygenated echinocytes. The effects may arise from a reduced level of intracellular adenosine triphosphate in echinocytic cells and an increased fraction of submembrane Hb.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Aniket Chowdhury, Raktim Dasgupta, Shovan K. Majumder, "Changes in hemoglobin–oxygen affinity with shape variations of red blood cells," Journal of Biomedical Optics 22(10), 105006 (20 October 2017). https://doi.org/10.1117/1.JBO.22.10.105006 . Submission: Received: 9 April 2017; Accepted: 25 September 2017
Received: 9 April 2017; Accepted: 25 September 2017; Published: 20 October 2017
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