5 October 2017 Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation
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J. of Biomedical Optics, 22(10), 106001 (2017). doi:10.1117/1.JBO.22.10.106001
Abstract
We report the results of characterization of red blood cell (RBC) structure and its dynamics with nanometric sensitivity using transport of intensity equation microscopy (TIEM). Conventional transport of intensity technique requires three intensity images and hence is not suitable for studying real-time dynamics of live biological samples. However, assuming the sample to be homogeneous, phase retrieval using transport of intensity equation has been demonstrated with single defocused measurement with x-rays. We adopt this technique for quantitative phase light microscopy of homogenous cells like RBCs. The main merits of this technique are its simplicity, cost-effectiveness, and ease of implementation on a conventional microscope. The phase information can be easily merged with regular bright-field and fluorescence images to provide multidimensional (three-dimensional spatial and temporal) information without any extra complexity in the setup. The phase measurement from the TIEM has been characterized using polymeric microbeads and the noise stability of the system has been analyzed. We explore the structure and real-time dynamics of RBCs and the subdomain membrane fluctuations using this technique.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Praveen Kumar Poola, Renu John, "Label-free nanoscale characterization of red blood cell structure and dynamics using single-shot transport of intensity equation," Journal of Biomedical Optics 22(10), 106001 (5 October 2017). http://dx.doi.org/10.1117/1.JBO.22.10.106001 Submission: Received 18 April 2017; Accepted 23 August 2017
Submission: Received 18 April 2017; Accepted 23 August 2017
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KEYWORDS
Microscopy

Blood

Absorption

3D image processing

3D image reconstruction

Microscopes

Refractive index

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