13 October 2017 Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos
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Abstract
Ramification of blood circulation is relevant in a number of physiological and pathological conditions. The oxygen exchange occurs largely in the capillary bed, and the cancer progression is closely linked to the angiogenesis around the tumor mass. Optical microscopy has made impressive improvements in in vivo imaging and dynamic studies based on correlation analysis of time stacks of images. Here, we develop and test advanced methods that allow mapping the flow fields in branched vessel networks at the resolution of 10 to 20    μ m . The methods, based on the application of spatiotemporal image correlation spectroscopy and its extension to cross-correlation analysis, are applied here to the case of early stage embryos of zebrafish.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Nicolo G. Ceffa, Nicolo G. Ceffa, Ilaria Cesana, Ilaria Cesana, Maddalena Collini, Maddalena Collini, Laura D’Alfonso, Laura D’Alfonso, Silvia Carra, Silvia Carra, Franco Cotelli, Franco Cotelli, Laura Sironi, Laura Sironi, Giuseppe Chirico, Giuseppe Chirico, } "Spatiotemporal image correlation analysis of blood flow in branched vessel networks of zebrafish embryos," Journal of Biomedical Optics 22(10), 106008 (13 October 2017). https://doi.org/10.1117/1.JBO.22.10.106008 . Submission: Received: 17 July 2017; Accepted: 26 September 2017
Received: 17 July 2017; Accepted: 26 September 2017; Published: 13 October 2017
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