Analysis of red blood cell parameters by Talbot-projected fringes

Shilpi Agarwal; Varun Kumar; Chandra Shakher

doi:10.1117/1.JBO.22.10.106009

Abstract

Red blood cell (RBC) anomalies are significant symptoms for identification of health disorders and several blood diseases, which involve the modification of the parameters and biophysical characteristics of such cells. The aim of this study is to measure the three-dimensional phase information of healthy RBCs and their parameters, such as cell diameter, thickness, and hemoglobin (Hb) content, using Talbot-projected fringes. The Talbot image of linear grating is projected onto an RBC slide. The deformed grating lines due to the shape and refractive index of RBCs are recorded by a CCD camera through a

20 \times

microscope objective. Hilbert transform is used to extract the phase image from the deformed projected grating lines. Experimentally calculated values of diameter (

8.2 μ m

), thickness (

2.7 μ m

), and Hb content (

28.7 pg / cell

) are well within the limits available in the literature. The proposed system is robust and user-friendly and performs the imaging of RBCs with high axial and lateral resolution (

2.19 μ m

Citation Download Citation

Shilpi Agarwal, Varun Kumar, Chandra Shakher, "Analysis of red blood cell parameters by Talbot-projected fringes," Journal of Biomedical Optics 22(10), 106009 (13 October 2017). https://doi.org/10.1117/1.JBO.22.10.106009 . Submission: Received: 16 June 2017; Accepted: 22 September 2017

Received: 16 June 2017; Accepted: 22 September 2017; Published: 13 October 2017

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