27 October 2017 Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue
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Abstract
Plastic embedding is widely applied in light microscopy analyses. Previous studies have shown that embedding agents and related techniques can greatly affect the quality of biological tissue embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial embedding resins and related embedding techniques directly. Here, we developed a neutral embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Hongfu Zhou, Hongfu Zhou, Yadong Gang, Yadong Gang, Shenghua Chen, Shenghua Chen, Yu Wang, Yu Wang, Yumiao Xiong, Yumiao Xiong, Longhui Li, Longhui Li, Fangfang Yin, Fangfang Yin, Yue Liu, Yue Liu, Xiuli Liu, Xiuli Liu, Shaoqun Zeng, Shaoqun Zeng, } "Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue," Journal of Biomedical Optics 22(10), 106015 (27 October 2017). https://doi.org/10.1117/1.JBO.22.10.106015 . Submission: Received: 5 July 2017; Accepted: 2 October 2017
Received: 5 July 2017; Accepted: 2 October 2017; Published: 27 October 2017
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