29 August 2017 Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery
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Abstract
The emergence of fluorescently labeled therapeutic antibodies has given rise to molecular probes for image-guided surgery. However, the extraneous interstitial presence of an unbound and nonspecifically accumulated probe gives rise to false-positive detection of tumor tissue and margins. Thus, the concept of tumor-cell activation of smart probes provides a potentially superior mechanism of delineating tumor margins as well as small tumor deposits. The combination of molecular targeting with intracellular activation circumvents the presence of extracellular, nonspecific signals of targeted probe accumulation. Here, we present a demonstration of the clinical antibodies cetuximab (cet, anti-EGFR mAb) and trastuzumab (trast, anti-HER-2 mAb) conjugated to Alexa Fluor molecules and IRDye QC-1 quencher optimized at the ratio of
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Girgis Obaid, Bryan Q. Spring, Shazia Bano, Tayyaba Hasan, "Activatable clinical fluorophore-quencher antibody pairs as dual molecular probes for the enhanced specificity of image-guided surgery," Journal of Biomedical Optics 22(12), 121607 (29 August 2017). https://doi.org/10.1117/1.JBO.22.12.121607 . Submission: Received: 6 May 2017; Accepted: 1 August 2017
Received: 6 May 2017; Accepted: 1 August 2017; Published: 29 August 2017
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