5 December 2017 Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue
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Abstract
The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [ T c ( t ) ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient ( D gl ) of glycerol in liver were found as 60.0% and 8.2 × 10 7    cm 2 / s , respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [ T c ( t ) ] and thickness measurements are performed during treatment. The knowledge of D gl is also important for living tissue cryoprotection applications.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Isa Carneiro, Isa Carneiro, Sónia Carvalho, Sónia Carvalho, Rui Henrique, Rui Henrique, Luís Oliveira, Luís Oliveira, Valery V. Tuchin, Valery V. Tuchin, } "Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue," Journal of Biomedical Optics 22(12), 125002 (5 December 2017). https://doi.org/10.1117/1.JBO.22.12.125002 . Submission: Received: 30 September 2017; Accepted: 15 November 2017
Received: 30 September 2017; Accepted: 15 November 2017; Published: 5 December 2017
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