12 December 2017 Real-time detection of antibiotic activity by measuring nanometer-scale bacterial deformation
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Abstract
Diagnosing antibiotic-resistant bacteria currently requires sensitive detection of phenotypic changes associated with antibiotic action on bacteria. Here, we present an optical imaging-based approach to quantify bacterial membrane deformation as a phenotypic feature in real-time with a nanometer scale ( 9    nm ) detection limit. Using this approach, we found two types of antibiotic-induced membrane deformations in different bacterial strains: polymyxin B induced relatively uniform spatial deformation of Escherichia coli O157:H7 cells leading to change in cellular volume and ampicillin-induced localized spatial deformation leading to the formation of bulges or protrusions on uropathogenic E. coli CFT073 cells. We anticipate that the approach will contribute to understanding of antibiotic phenotypic effects on bacteria with a potential for applications in rapid antibiotic susceptibility testing.
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)
Rafael Iriya, Rafael Iriya, Karan Syal, Karan Syal, Wenwen Jing, Wenwen Jing, Manni Mo, Manni Mo, Hui Yu, Hui Yu, Shelley E. Haydel, Shelley E. Haydel, Shaopeng Wang, Shaopeng Wang, Nongjian Tao, Nongjian Tao, } "Real-time detection of antibiotic activity by measuring nanometer-scale bacterial deformation," Journal of Biomedical Optics 22(12), 126002 (12 December 2017). https://doi.org/10.1117/1.JBO.22.12.126002 . Submission: Received: 5 July 2017; Accepted: 17 November 2017
Received: 5 July 2017; Accepted: 17 November 2017; Published: 12 December 2017
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